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KMID : 0385520030160010078
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Analytical Science & Technology
2003 Volume.16 No. 1 p.78 ~ p.81
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A study on the optimal HPLC condition for peptides complex analysis using mass spectrometry
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권성원/Kwon SW
박철홍/Park CH
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Abstract
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Peptides separation in high performance liquid chromatography (HPLC) is very important for the analysis of total proteins using mass spectrometry rather than two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In this study, we investigated the optimal HPLC condition of peptides for the use of mass fingerprinting. As a result of pursuing a combination of solvent additives for HPLC, water and acetonitile containing both 0.1% trifluoroacetic acid and 0.1% acetic acid respectively showed the most efficient resolution and sensitivity.
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